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我的位置:首頁  >  產(chǎn)品中心  >  生化試劑  >  AnaSpec  >  AS-72113AnaSpec AnaTag™ R-PE標(biāo)簽試劑盒

AnaSpec AnaTag™ R-PE標(biāo)簽試劑盒

  • 產(chǎn)品型號:AS-72113
  • 更新時間:2024-10-09

簡要描述:產(chǎn)品簡介:
產(chǎn)品品牌:AnaSpec
產(chǎn)品名稱:AnaTag™ R-PE標(biāo)簽試劑盒-1試劑盒
英文名稱:AnaTag™ R - PE Labeling Kit - 1 kit
產(chǎn)品貨號:AS-72113
產(chǎn)品規(guī)格:1 kit
AnaSpec AnaTag™ R-PE標(biāo)簽試劑盒

產(chǎn)品詳情
品牌AnaSpec貨號AS-72113
規(guī)格1 kit供貨周期現(xiàn)貨

AnaSpec AS-72113 AnaTag™ R - PE Labeling Kit - 1 kit,AnaTag™ R-PE標(biāo)簽試劑盒-1試劑盒

我們北京云肽生物科技有限公司作為AnaSpec公司的經(jīng)銷商為客戶提供,正規(guī)進(jìn)口,保證原裝正品,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。

AnaSpec ( 美國 )

AnaSpec公司成立于1993年,是一家為全球生命科學(xué)研究,提供綜合蛋白質(zhì)組學(xué)和基因組學(xué)解決方案的領(lǐng)xian供應(yīng)商。
公司產(chǎn)品主要分三大部分:提供專業(yè)多肽,檢測試劑,抗體檢測試劑盒;適合基礎(chǔ)研究的高通量篩選和新藥物研發(fā)相關(guān)試劑;多肽合成,抗體生產(chǎn),寡核苷酸的合成等定制服務(wù)。

AnaSpec AS-72113 AnaTag™ R - PE Labeling Kit - 1 kit,AnaTag™ R-PE標(biāo)簽試劑盒-1試劑盒,產(chǎn)品簡介:

產(chǎn)品品牌:AnaSpec

產(chǎn)品名稱:AnaTag™ R-PE標(biāo)簽試劑盒-1試劑盒

英文名稱:AnaTag™ R - PE Labeling Kit - 1 kit

產(chǎn)品貨號:AS-72113

產(chǎn)品規(guī)格:1 kit

AnaSpec AnaTag™  R-PE標(biāo)簽試劑盒

AnaSpec AS-72113 AnaTag™ R - PE Labeling Kit - 1 kitAnaTag™ R-PE標(biāo)簽試劑盒-1試劑盒,產(chǎn)品說明:

The AnaTag™ R-PE Labeling Kit is optimized for use in the conjugation of R-Phycoerythrin (R-PE) to antibodies. R-PE, a fluorescent protein from phycobiliprotein family, has broad absorption bands with peaks at 565 nm (eM =1.96 X 106 M-1cm-1), 498 (eM =1.53 X 106 M-1cm-1), and 539 nm (eM =1.62 X 106 M-1cm-1); it therefore can be excited with versatile excitation sources. Its maximal emission can be detected at 578 nm. The broad excitation spectrum also provides the advantage of multi-color immunofluorescent staining or cell sorting. R-PE and the closely related B-PE are the most intensely fluorescent phycobiliproteins having orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. The AnaTagTM R-PE Labeling Kit contains SH-reactive R-PE. SMCC modified R-PE reacts with the thiol groups of target antibody without the need for additional activation, thus simplifying the conjugation protocol. The amount of R-PE supplied in this kit is sufficient for labeling up to 1 mg of antibody. The kit provides all reagents, purification columns and a detailed step-by-step protocol. Native and SMCC-activated R-PE are also available as separate products.

Note: AnaTag kits are not intended for peptide labeling.

The AnaTag™ R-PE標(biāo)記試劑盒經(jīng)過優(yōu)化,可用于R-藻紅蛋白(R-PE)與抗體的結(jié)合。R-PE是藻膽蛋白家族的一種熒光蛋白,具有寬的吸收帶,峰值為565nm(eM=1.96x106m-1cm-1)、498(eM=1.53x106m-1cm-1)和539nm(eM=1.62x106m-1cm-1);因此,可以使用多種激勵源對其進(jìn)行激勵。其最大發(fā)射波長為578nm。寬激發(fā)光譜還提供了多色免疫熒光染色或細(xì)胞分選的優(yōu)勢。R-PE和密切相關(guān)的B-PE是熒光zui強(qiáng)的藻膽蛋白,具有橙色熒光。它們比傳統(tǒng)的有機(jī)熒光團(tuán)更明亮、更耐光。AnaTagTM R-PE標(biāo)記試劑盒包含SH反應(yīng)性R-PE。SMCC修飾的R-PE與目標(biāo)抗體的巰基反應(yīng),無需額外活化,從而簡化接合方案。該試劑盒中提供的R-PE量足以標(biāo)記高達(dá)1mg的抗體。該試劑盒提供所有試劑、純化柱和詳細(xì)的分步方案。天然和SMCC活化R-PE也可作為單獨(dú)的產(chǎn)品提供。

注:AnaTag試劑盒不用于肽標(biāo)記。

成分A:活化R-PE(SMCC活化交聯(lián)R-藻紅蛋白):3 mg,10 mg/mL組分B:DTT:0.1 mL組分C:旋轉(zhuǎn)柱:1柱組分D:洗滌管:1管組分E:收集管:1管組分F:重力柱:1柱組分G:DMSO:1 mL組分H:NEM以阻斷游離巰基:1小瓶組分I:用于B-PE與蛋白質(zhì)接合的反應(yīng)緩沖液:100 mL

AnaSpec AnaTag™  R-PE標(biāo)簽試劑盒

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